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Disease Markers
Volume 34 (2013), Issue 3, Pages 187-197

Association between Endothelial Nitric Oxide Synthase Polymorphisms and Risk of Metabolic Syndrome

Chiu-Shong Liu,1,2 Ru-Jiun Huang,3,4 Fung-Chang Sung,5 Cheng-Chieh Lin,1,2,6 and Chih-Ching Yeh3,4,5

1Department of Family Medicine, China Medical University Hospital, Taichung, Taiwan
2School of Medicine, College of Medicine, China Medical University, Taichung, Taiwan
3Department of Health Risk Management, College of Public Health, China Medical University, Taichung, Taiwan
4School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan
5Department of Public Health, College of Public Health, China Medical University, Taichung, Taiwan
6Department of Healthcare Administration, College of Health Science, Asia University, Taichung, Taiwan

Received 14 January 2013; Accepted 14 January 2013

Copyright © 2013 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


BACKGROUND: Previous studies inferring that the NOS3 gene was associated with the pathogenesis of metabolic syndrome (MetS) had inconsistent findings. We investigated the role of three NOS3 polymorphisms (T-786C, intron 4b/a, and G894T) in the risk of MetS using a hospital-based case-control study.

METHODS: We recruited 339 MetS cases and 783 non-MetS controls at a central Taiwanese hospital. Information on sociodemographic and lifestyle factors was obtained using a self-administered questionnaire. Genotypes of NOS3 polymorphisms were compared between cases and controls. Effects of interactions between gene polymorphisms and smoking and between gene polymorphisms and drinking on the risk of MetS were also determined.

RESULTS: The T-786C TC+CC genotype was significantly associated with a decreased risk of MetS (odds ratio (OR), 0.63; 95% confidence interval (CI), 0.43–0.91), compared to the T-786C TT genotype, according to a logistic regression analysis. This beneficial effect was much greater for those who had ever smoked cigarettes (OR, 0.47; 95% CI, 0.26–0.87) or those who had not consumed alcohol (OR, 0.45; 95% CI, 0.26–0.77). In addition, the intron 4b/a variant genotype was marginally associated with a reduced risk of MetS (OR, 0.68; 95% CI, 0.47–1.00), compared to the intron 4b/a bb genotype, particularly for never alcohol consumers (OR, 0.56; 95% CI, 0.33–0.95). In the haplotype analysis, there was a 53% decrease in the MetS risk among C4bG haplotype carriers (OR, 0.47; 95% CI, 0.25–0.90), compared to those with the most common T4bG haplotype.

CONCLUSIONS: Our results suggest that the NOS3 T-786C and intron 4b/a polymorphisms may contribute to the risk of MetS. Further studies are needed to confirm the findings.