Evaluation of Molecular Species of Prostate-Specific Antigen Complexed with Immunoglobulin M in Prostate Cancer and Benign Prostatic Hyperplasia
Figure 2
Representative spectra of PSA-immunoreactive species complexed with IgM. On-chip chromatography was performed using anti-fPSA monoclonal antibody array. Corresponding samples were incubated for 2 h in a humid chamber at RT. The spots were rinsed, and, after drying, 50% sinapinic acid was added to each spot, dried, and then reapplied. The spectra were obtained by SELDI-TOF/MS. The -axis represents the distribution of ions by mass (mass to charge ratio, ) and the -axis gives the signal intensity of the ions. M + 2H: double charged ion. (a) PCa PSA-IgM (PSA-IgM from PCa sera); (b) BPH PSA-IgM (PSA-IgM from BPH sera); (c) IgM, control sample. Peaks found on the control chip were considered as nonspecific. The region below 12 kDa is not shown since it was obscured by non-specific binding. Highlighted peak at 26889.27 Da was detected only in PCa samples.
