Altered Peptidase Activities in Thyroid Neoplasia and Hyperplasia

Table 2

(a) Peptidase activities in papillary thyroid carcinoma (PTC) and in non neoplastic adjacent thyroid tissue. (b) Peptidase activities in follicular thyroid adenoma (FTA) and in non neoplastic adjacent thyroid tissue. (c) Peptidase activities in thyroid nodular hyperplasia (TNH) and in non neoplastic adjacent thyroid tissue. Values are means ± SE of peptidase activity recorded as pmol of units of peptidase (UP) per milligram of protein. A value of 𝑃≤0.05 was considered statistically significant (Significant results are in bold).

(a)

Peptidase	PTC	Non neoplastic	P
Soluble fraction
APB	7419 ± 1363	4259 ± 867	0.094
AspAP	130 ± 15	87 ± 13	0.039
PG I	206 ± 39	77 ± 18	0.008
PEP	3836 ± 633	1977 ± 449	0.026
AlaAP	8819 ± 1194	4122 ± 1111	0.007
Membrane-bound fraction
APA	1250 ± 280	1120 ± 258	0.96
APN/CD13	2301 ± 538	2533 ± 516	0.554
CAP	875 ± 253	665 ± 180	0.425
DPPIV/CD26	4274 ± 1301	1347 ± 278	0.017
NEP/CD10	3.1 ± 0.66	1.9 ± 0.45	0.12

(b)

Peptidase	FTA	Non neoplastic	P
Soluble fraction
APB	3772 ± 318	1969 ± 210	0.001
AspAP	84 ± 7.2	54 ± 21	0.003
PG I	245 ± 20	85 ± 12	0.001
PEP	3056 ± 359	1482 ± 184	0.002
AlaAP	2022 ± 206	792 ± 76	0.001
Membrane-bound fraction
APA	1513 ± 191	1270 ± 141	0.247
APN/CD13	1670 ± 198	2542 ± 274	0.105
CAP	453 ± 48	407 ± 47	0.354
DPPIV/CD26	589 ± 87	1700 ± 546	0.037
NEP/CD10	3.7 ± 0.63	2.8 ± 0.45	0.643

(c)

Peptidase	TNH	Nonhyperplastic	P
Soluble fraction
APB	3713 ± 688	5477 ± 721	0.054
AspAP	67 ± 4.3	66 ± 14.4	0.519
PG I	73 ± 7.8	81 ± 5.3	0.687
PEP	2934 ± 448	3580 ± 478	0.295
AlaAP	1834 ± 258	2087 ± 310	0.227
Membrane-bound fraction
APA	789 ± 140	475 ± 87	0.116
APN/CD13	1775 ± 329	1856 ± 243	0.601
CAP	317 ± 73	394 ± 107	0.687
DPPIV/CD26	319 ± 44	804 ± 198	0.116
NEP/CD10	1.34 ± 0.25	1.74 ± 0.29	0.414