miR-146a Expression Level as a Novel Putative Prognostic Marker for Acute Promyelocytic Leukemia

Figure 2

Exogenous miR-146a and endogenous Smad4 expression. (a) Western blot analysis of Smad4 protein expression in NB₄ cells after transfection with miR-146a duplex, miR-146a inhibitor duplex, or scrambled duplex. (b) RTq-PCR analysis of Smad4 gene expression in NB₄ cells after transfection with miR-146a duplex, miR-146a inhibitor duplex, or scrambled duplex. (c) miR-146a was inversely correlated with Smad4 protein expression in samples from 14 APL patients. (d) miR-146a and Smad4 protein expression analysis in samples from 6 APL patients at diagnosis and after complete remission using RTq-PCR and western blotting. Normalized mean values of 3 independent experiments and standard errors (means ± SDs) are shown. ∗, 𝑃≤0.05; ∗∗, 𝑃≤0.001 (paired 𝑡 test).

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