Proteome Changes in the Plasma of Myelodysplastic Syndrome Patients with Refractory Anemia with Excess Blasts Subtype 2

Table 1

List of spots that differed significantly when RAEB-2 patients were compared with healthy controls.

Spot	𝑃	Fold	Protein	Peptides	AC	SC (%)
1	0.0002	−3.8	Serum albumin	20	P02768	56
			Fibrinogen alpha chain	2	P02671	3
			Hemopexin	2	P02790	20
2	0.008	−3.3	Serum albumin	5	P02768	18
3	0.001	−2.8	Serum albumin	18	P02768	46
			Hemopexin	3	P02790	23
4	0.004	−2.7	Plasma protease C1 inhibitor	5	P05155	15
5	0.0001	−2.4	Alpha-2-HS glycoprotein	2	P02765	17
			Kininogen-1	3	P01042	7
			Corticosteroid-binding globulin	2	P08185	14
6	0.003	−2.4	Serum albumin	5	P02768	13
7	0.007	−2.4	Serum albumin	12	P02768	31
8	0.004	−2.2	Serum albumin	22	P02768	49
9	0.0005	−2.2	Serum albumin	24	P02768	59
10	0.024	2.1	Apolipoprotein A-I	2	P02647	8
			C-reactive protein	4	P02741	20
11	0.008	−2.1	Serum albumin	15	P02768	43
			Protein Z-dependent protease inhibitor	2	Q9UK55	5
12	0.016	2.1	Unidentified
13	0.017	1.9	Apolipoprotein A-IV	3	P06727	12
			Complement factor I	2	P05156	8
14	0.002	−1.9	Inter-alpha-trypsin inhibitor heavy chain H4	5	Q14624	11
15	0.02	1.9	Serum albumin	6	P02768	16
			Prothrombin	4	P00734	17
			Histidine-rich glycoprotein	2	P04196	4
16	0.001	−1.9	Alpha-2-HS glycoprotein	2	P02765	15
			Kininogen-1	2	P01042	4
17	0.011	−1.9	Serum albumin	11	P02768	26
			Hemopexin	2	P02790	16
18	0.022	−1.9	Serum albumin	7	P02768	13
19	0.0003	1.8	Leucine-rich alpha-2-glycoprotein	7	P02750	30
			Alpha-1-antichymotrypsin	2	P01011	8
20	0.003	1.8	Leucine-rich alpha-2-glycoprotein	5	P02750	28
21	0.039	−1.7	Unidentified
22	0.002	−1.7	Serum albumin	3	P02768	12
23	0.029	1.7	Haptoglobin	4	P00738	18
24	0.004	1.7	Inter-alpha-trypsin inhibitor heavy chain H4	13	Q14624	20
			Alpha-1-antitrypsin	7	P01009	37
			Angiotensinogen	2	P01019	9
			Prothrombin	2	P00734	14
25	0.006	−1.6	Serum albumin	5	P02768	14
26	0.01	−1.6	Unidentified
27	0.027	−1.6	Inter-alpha-trypsin inhibitor heavy chain H4	5	Q14624	6
28	0.011	−1.6	Serum albumin	5	P02768	11
			Coagulation factor XII	3	P00748	12
29	0.0008	1.6	Ficolin-2	1	Q15485	4
30	0.02	−1.5	Serum albumin	4	P02768	13
			Apolipoprotein A-IV	2	P06727	7
31	0.0006	−1.5	Clusterin	3	P10909	12
32	0.001	−1.5	Unidentified
33	0.004	−1.5	Clusterin	2	P10909	7
34	0.016	−1.5	Beta-2-glycoprotein 1	3	P02749	21
35	0.002	−1.5	Prothrombin	7	P00734	26
			Inter-alpha-trypsin inhibitor heavy chain H4	2	Q14624	6
			Alpha-1B-glycoprotein	2	P04217	9
36	0.009	−1.4	Tetranectin	4	P05452	37
37	0.002	−1.4	Tetranectin	2	P05452	16
38	0.0002	1.4	Alpha-1-antichymotrypsin	12	P01011	47
			Corticosteroid-binding globulin	3	P08185	11
39	0.014	−1.4	Alpha-1-antichymotrypsin	3	P01011	11
			Inter-alpha-trypsin inhibitor heavy chain H4	3	Q14624	6
			Clusterin	4	P10909	14
40	0.003	−1.4	Clusterin	4	P10909	14
41	0.017	1.4	Alpha-1-antichymotrypsin	2	P01011	8
			Zinc-alpha-2 glycoprotein	2	P25311	9
42	0.03	−1.3	Inter-alpha-trypsin inhibitor heavy chain H4	4	Q14624	8
43	0.002	−1.3	Clusterin	4	P10909	15
44	0.027	1.3	Inter-alpha-trypsin inhibitor heavy chain H4	13	Q14624	19
			Alpha-1-antitrypsin	3	P01009	18
			Complement component C9	2	P02748	6
45	0.012	−1.3	Clusterin	3	P10909	17
			Inter-alpha-trypsin inhibitor heavy chain H4	2	Q14624	3
46	0.037	−1.3	Clusterin	4	P10909	17
			Inter-alpha-trypsin inhibitor heavy chain H4	3	Q14624	4
47	0.045	1.2	Protein AMBP	2	P02760	12

𝑃: ANOVA 𝑃 value; fold: fold difference (multiplication) when comparing RAEB-2 patients and healthy controls (+fold when the normalized volumes increased in RAEB-2); protein: protein identification; peptides: number of unique peptides fulfilling a minimal Mascot score for identity; AC: accession number (SWISS-PROT); SC: protein sequence coverage.