Comparative Plasma Protein Profiling of Hemoglobin H Disease

Table 2

List of 17 differential spots representing 14 different plasma proteins compared between normal control, HbH, and HbH-CS.

Number	Name of protein	MW*	pI**	MOWSE score	Sequence coverage (%)	Intensity ratio of normal control/patient	𝑃 value	Tissue specificity	Functions
H01	Gelsolin	86043	5.9	72	14	2.26	0.0141	Phagocytic cells, platelets, fibroblasts, muscle cells	actin-binding proteins,; calcium-regulated protein
H02	Serotransferrin	79294	6.81	1180	54	1.56	0.028	Liver	Iron binding transport proteins
H03	Complement C3	79294	6.02	272	11	2.89	0.037	Liver	Phagocytosis, inflammatory responses, activation cell damage
H04	Alpha-1-antitrypsin	46878		553	37	0.72	0.044	Liver	Irreversible inhibition of trypsin
H05	Ig gamma-1 chain C region	36596	8.46	58	16	0.2	0.003	B lymphocyte	Immune response
H06	Ig gamma-2 chain C region	36505	7.66	42	16	0.38	0.015	B lymphocyte	Immune response
H10	Apolipoprotein A-I	30759	5.56	696	73	1.65	0.003	Liver	Transport of cholesterol
H11	Haptoglobin	45861	6.13	172	13	3.15	0.021	Liver	Hb-scavenging proteins,; hemolytic marker
H12	Haptoglobin	45861	6.13	172	13	3.6	0.007
H13	Haptoglobin	45861	6.13	102	13	3.64	0.013
H14	Transthyretin	15991	5.52	325	34	3.34	0.018	Liver	Thyroxin-binding prealbumin, redox reaction
H15	Ig mu chain C region	49960	6.35	46	10	0.32	0.007	B lymphocyte	Immune response
H08	Actin	42052	5.29	141	29	4.82	0.005	Cell membrane	Red-cell skeleton membrane protein
H09	Haptoglobin-related protein	39518	5.92	115	24	3.13	0.05	Liver	Hb binding protein
H07	Serum paraoxonase	39877	5.08	72	17	4.06	0.009	Liver	Antioxidant, detoxification of organophosphates
H16	Serum albumin	71317	5.92	363	20	0.50	0.010	Liver	Regulation of the colloidal osmotic pressure of blood
H17	Serum albumin	71317	5.92	180	17	0.45	0.014

∗∗pI: isoelectric point and *MW: molecular weight.