Promoter Methylation of SFRP3 Is Frequent in Hepatocellular Carcinoma
Figure 1
Methylation of SFRP3 in primary hepatocellular carcinoma tissues. (a) Schematic representation of the promoter region and the first exon of the SFRP3 gene. The CpG rich areas and the sites of methylation specific PCR (MSP), quantitative MSP, and bisulfite sequencing (BS) primers are indicated. (b) Representative results for four control livers (N1 to N5), four HCCs (T), and their corresponding nontumor livers (NT). Bisulfite-modified genomic DNA was amplified using methylation-specific or unmethylation-specific primer sets. M, methylation-specific PCR product; U, unmethylation-specific PCR. DNA from the peripheral blood lymphocyte (PBL) sample was used as a negative control, and PBL DNA treated with SssI Methylase (New England Biolabs, Beverly, MA) was a positive control. Case numbers are indicated at the top. (c) Summary of bisulfite sequencing. Case numbers of tumors, nontumor tissues, and normal control are indicated at the top. Black and white circles correspond to methylated or unmethylated, respectively.
