Research Article

Promoter Methylation of SFRP3 Is Frequent in Hepatocellular Carcinoma

Figure 2

Promoter methylation and downregulation of SFRP3 in HCC cell lines. (a) Detection of methylation in HCC cell lines using MS-PCR. M, methylation-specific PCR product; U, unmethylation-specific PCR. Four cell lines were treated for 4 days with the indicated concentration of 5-Aza-CdR. MS-PCR assay on DNA isolated from untreated or treated HCC cells. (b) Summary of bisulfite sequencing. The name of HCC cell line is indicated at the top. Black and white circles correspond to methylated or unmethylated, respectively. (c) HCC cell lines were treated with 5-aza-2′-deoxycytidine (5-Aza-CdR, DAC) for 4 days. The mRNA of SFRP3 was analyzed by Q-RT-PCR. Expression of GAPDH was determined as a control for RNA quality. Significant differences were analyzed using the Mann-Whitney test (* for and *** for ).
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