Promoter Methylation of SFRP3 Is Frequent in Hepatocellular Carcinoma

Figure 3

Frequent downregulation of SFRP3 is associated with promoter hypermethylation in primary HCCs. The SFRP3 transcripts of 60 primary HCCs (T) and their corresponding adjacent nontumor tissues (NT) were analyzed by RT-PCR and normalized to the internal control (GAPDH). Next, the methylation status of clinical HCC tissues was checked by QMSP and normalized to the internal reference gene COL2A. Significant differences were analyzed using the paired sample 𝑡-test or Mann-Whitney 𝑈 test (* for 𝑃<0.05 and *** for 𝑃<0.001).

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