Promoter Methylation of SFRP3 Is Frequent in Hepatocellular Carcinoma
Table 2
SFRP3 mRNA expression in primary HCCs by relative quantitative RT-PCR.
Patient no.
SFRP3 methylation
Ct
Ct
SFRP3 tumor part
SFRP3-GAPDH
Ct tumor − Ct nontumor
Rel. to nontumor
1T
U
9.03
1.68
0.3121
1NT
U
7.35
2T
M
10.05
2.81
0.1426
2NT
M
7.24
3T
U
7.63
−0.47
1.3851
3NT
U
8.10
4T
U
7.58
−0.51
1.4191
4NT
U
8.09
5T
U
11.54
0.82
0.5684
5NT
U
10.72
6T
U
5.92
−0.29
1.2226
6NT
U
6.21
7T
M
7.40
1.38
0.3856
7NT
U
6.03
8T
U
15.00
6.10
0.0146
8NT
U
8.91
9T
M
8.95
1.91
0.2661
9NT
U
7.04
10T
M
9.03
1.79
0.2892
10NT
M
7.24
11T
M
15.00
9.03
0.0019
11NT
M
5.97
12T
M
9.10
1.35
0.3923
12NT
M
7.75
13T
U
9.62
1.58
0.3356
13NT
U
8.04
14T
U
6.27
−0.71
1.6358
14NT
U
6.98
15T
M
15.00
7.90
0.0042
15NT
U
7.10
16T
M
15.00
7.14
0.0071
16NT
M
7.86
17T
M
9.34
1.13
0.4569
17NT
M
8.21
18T
U
5.10
−1.01
2.0069
18NT
U
6.11
19T
M
6.75
1.04
0.4863
19NT
M
5.71
20T
U
15.00
7.87
0.0043
20NT
U
7.14
21T
M
15.00
8.13
0.0036
21NT
U
6.87
22T
U
9.92
3.48
0.0899
22NT
U
6.45
23T 23NT
M M
9.05 7.63
1.42
0.3737
24T
M
8.47
1.24
0.4248
24NT
M
7.23
25T
M
6.96
0.61
0.6552
25NT
M
6.35
26T
U
5.14
0.11
0.9298
26NT
U
5.04
27T
M
12.37
5.31
0.0253
27NT
M
7.06
28T
M
15.00
6.21
0.0136
28NT
U
8.80
29T
U
5.67
2.49
0.1780
29NT
U
3.18
30T
M
9.23
1.44
0.3680
30NT
U
7.79
31T
U
15.00
6.34
0.0123
31NT
U
8.66
32T
U
8.28
1.18
0.4429
32NT
U
7.11
33T
U
12.14
5.96
0.0161
33NT
U
6.18
34T
U
7.63
2.47
0.1811
34NT
U
5.16
35T
M
6.98
4.65
0.0398
35NT
M
2.33
36T
M
15.00
5.01
0.0310
36NT
M
9.99
37T
U
15.00
9.40
0.0015
37NT
U
5.61
38T
M
15.00
10.72
0.0006
38NT
M
4.28
39T
M
7.90
1.10
0.4665
39NT
U
6.80
40T
M
15.00
7.48
0.0056
40NT
U
7.52
41T
M
8.97
1.22
0.4308
41NT
U
7.75
42T
M
9.25
2.17
0.2222
42NT
U
7.08
43T
U
15.00
8.77
0.0023
43NT
U
6.23
44T
M
8.76
0.22
0.8586
44NT
M
8.54
45T
M
8.92
2.86
0.1377
45NT
U
6.06
46T 46NT
U U
10.34 8.17
2.17
0.2222
47T
M
15.00
9.13
0.0018
47NT
U
5.88
48T
U
15.00
9.16
0.0018
48NT
U
5.85
49T
M
15.00
9.43
0.0014
49NT
U
5.57
50T
M
15.00
6.78
0.0091
50NT
U
8.22
51T
M
15.00
6.16
0.0140
51NT
U
8.85
52T
M
15.00
10.77
0.0006
52NT
U
4.24
53T
U
11.21
3.77
0.0733
53NT
U
7.44
54T
M
12.27
10.28
0.0008
54NT
U
1.99
55T
M
15.00
4.94
0.0326
55NT
U
10.06
56T
U
7.74
1.98
0.2535
56NT
U
5.76
57T
U
10.39
3.52
0.0872
57NT
U
6.87
58T
M
15.00
9.01
0.0019
58NT
U
5.99
59T
M
11.36
3.99
0.0632
59NT
U
7.38
60T
M
7.52
2.55
0.1713
60NT
U
4.97
NT: nontumor part; T: tumor part; M: methylated; U: unmethylated. The range given for SFRP3 tumor part relative to nontumor part is determined by evaluating the expression: 2−ΔΔCt.
