Prognostic and Biological Significance of MicroRNA-127 Expression in Human Breast Cancer

Figure 3

Upregulation of miR-127 expression inhibits growth, reduces colony formation, and enhances apoptosis in BC cells. (a) qRT-PCR analysis of miR-127 expression in stably transfected MCF-7/miR-127 (or MCF-7/miR-NC) and SK-BR-3/miR-127 (SK-BR-3/miR-NC) cells. U6 was used as an internal control. (b) MTT analysis of growth in MCF-7/miR-127 (or MCF-7/miR-NC) and SK-BR-3/miR-127 (or SK-BR-3/miR-NC) cells. (c) The colony formation assay was performed as described in Materials and Methods section. The number of colonies was counted and compared. (d) Flow cytomeric detection of apoptosis in MCF-7/miR-127 (or MCF-7/miR-NC) and SK-BR-3/miR-127 (or SK-BR-3/miR-NC) cells. (e) Western blot analysis of the expression levels of cleaved caspase-3, total caspase-3, cleaved PARP, and total PARP proteins. GAPDH was used as an internal control. Each experiment was performed at least in triplicate. , versus control.

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