MicroRNA-224 Suppresses Colorectal Cancer Cell Migration by Targeting Cdc42

Figure 3

Increased miR-224 expression diminished Cdc42 and SMAD4 expressions and inhibited the formation of actin filaments. (a) Prediction of miR-224 targeting mRNA sequences on SMAD4 and Cdc42 in the Sloan-Kettering Cancer Center Human MicroRNA Targets Database. (b, c) mRNA and protein levels of SMAD4 and Cdc42 in NCM- and 224 M-transfected HCT-116 cells (*𝑃<0.05; **𝑃<0.01). (d) Representative Western blot of G- and F-actin from NCM- and 224 M-transfected HCT-116 cells. Data are from one of three experiments. (e, f) Migratory ability of Cdc42 silenced HCT-116 cells transfected with NCM and 224 M (*𝑃<0.05, compared to TR alone).

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