Hypertrophy of Ligamentum Flavum in Lumbar Spine Stenosis Is Associated with Increased miR-155 Level
Figure 5
miR-155 sponge decreases the expression of collagen I and collagen III in fibroblasts isolated from LF. (a) Real-time PCR analysis showed that the relative mRNA levels of collagen I and collagen III were significantly lower in Sponge group than in Blank and Control groups. (b) Western blot analysis of collagen I and collagen III expression in different groups of fibroblasts. (c) The relative protein levels of collagen I and collagen III were significantly lower in Sponge group than in Blank and Control groups. (d) Blank group. (e) Control group. (f) Immunocytochemical staining of collagen I in Sponge group. (g) Immunocytochemical staining of collagen III in Sponge group. Brown indicated positive staining.
