Urine Annexin A1 as an Index for Glomerular Injury in Patients

Figure 3

Cellular expression of ANXA1 and ANXA2 mRNAs demonstrated by ISH at different time points after ADG induction. (a)–(f) ANXA1 and (g)–(l) ANXA2. Kidney tissue sections on day 0 ((a) and (g)), day 3 ((b) and (h)), day 7 ((c) and (i)), day 14 ((d) and (j)), or day 21 ((e) and (k)). The arrows indicate epithelial cells in the crescent-like formation of the glomerulus. Original magnification, ×400. (f) and (l) Semiquantitative analysis of cellular protein expression by ISH. Scoring was performed for the three major components of the parietal epithelial cells (solid bars), podocytes (open bars), and mesangial cells (hatched bars). Each bar represents the mean±SEM for six mice per group. ^∗𝑃<0.05; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.005, compared to the normal controls (day 0). #: not detectable.