Research Article
Polymorphisms in the IFNγ, IL-10, and TGFβ Genes May Be Associated with HIV-1 Infection
Table 1
Restriction enzymes, primer sequences, annealing temperatures, and fragments generated during polymorphism identification.
| Polymorphisms (endonuclease) | Primers | Genome location | Hybridization temperature (°C) | Alleles and fragments (bp) |
| rs1800795 (Nla III) | IL-6 F: 5′-AAAGGAAGAGTGGTTCTGCTTCT-3′ IL-6 R: 5′-ATCTTTGTTGGAGGGTGAGG-3′ | -174G/C (promoter) | 59 | C: 85, 84 G: 169 |
| rs1800896 (Mnl I) | IL-10 F: 5′-TCTGAAGAAGTCCTGATGTC-3′ IL-10 R: 5′-CTCTTACCTATCCCTACTTCC-3′ | -1082A/G (promoter) | 60 | A: 125, 65 G: 93, 65, 32 |
| rs2430561 (Hinf I) | INFg F: 5′-GATTTTATTCTTACAACACAAAATCAAGAC-3′ INFg R: 5′-GCAAAGCCACCCCACTATAA-3′ | +874T/A (first intron) | 53 | A: 176 T: 148, 28 |
| rs1800629 (Nco I) | TNFa F: 5′-AGGCAATAGGTTTTGAGGGCCAT-3′ TNFa R: 5′-TCCTCCCTGCTCCGATTCCG-3′ | -308G/A (promoter) | 60 | A: 107 G: 87, 20 |
| rs1800469 (Dde I) | TGFβ1 F: 5′-GGAGAGCAATTCTTACAGGTG-3′ TGFβ1 R: 5′-TAGGAGAAGGAGGGTCTGTC-3′ | -509C/T (promoter) | 61 | T: 120 C: 74, 46 |
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F: forward; R: reverse; bp: base pairs.
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