Polymorphisms in the IFNγ, IL-10, and TGFβ Genes May Be Associated with HIV-1 Infection

Table 1

Restriction enzymes, primer sequences, annealing temperatures, and fragments generated during polymorphism identification.

Polymorphisms; (endonuclease)	Primers	Genome location	Hybridization temperature; (°C)	Alleles and fragments (bp)
rs1800795; (Nla III)	IL-6 F: 5′-AAAGGAAGAGTGGTTCTGCTTCT-3′; IL-6 R: 5′-ATCTTTGTTGGAGGGTGAGG-3′	-174G/C (promoter)	59	C: 85, 84; G: 169
rs1800896; (Mnl I)	IL-10 F: 5′-TCTGAAGAAGTCCTGATGTC-3′; IL-10 R: 5′-CTCTTACCTATCCCTACTTCC-3′	-1082A/G (promoter)	60	A: 125, 65; G: 93, 65, 32
rs2430561; (Hinf I)	INFg F: 5′-GATTTTATTCTTACAACACAAAATCAAGAC-3′; INFg R: 5′-GCAAAGCCACCCCACTATAA-3′	+874T/A (first intron)	53	A: 176; T: 148, 28
rs1800629; (Nco I)	TNFa F: 5′-AGGCAATAGGTTTTGAGGGCCAT-3′; TNFa R: 5′-TCCTCCCTGCTCCGATTCCG-3′	-308G/A (promoter)	60	A: 107; G: 87, 20
rs1800469; (Dde I)	TGFβ1 F: 5′-GGAGAGCAATTCTTACAGGTG-3′; TGFβ1 R: 5′-TAGGAGAAGGAGGGTCTGTC-3′	-509C/T (promoter)	61	T: 120; C: 74, 46

F: forward; R: reverse; bp: base pairs.