Clinicopathological Significance of MicroRNA-20b Expression in Hepatocellular Carcinoma and Regulation of HIF-1α and VEGF Effect on Cell Biological Behaviour

Figure 10

The cells apoptosis detected by Annexin V-FITC/PI assay. HepG2 cells were transfected with miR-20b inhibitor, inhibitor + si-HIF-1α, and inhibitor + si-VEGF. Inhibitor + si-HIF-1α: miR-20b inhibitor + HIF-1α-siRNA; inhibitor + si-VEGF: miR-20b inhibitor + VEGF-siRNA. compared with control in normoxia (a). Transfected miR-20b mimics for 24 h in mimetic hypoxia conditions; apoptosis significantly increased in hypoxia conditions (b).

(a)

(b)