Hiwi Promotes the Proliferation of Colorectal Cancer Cells via Upregulating Global DNA Methylation

Figure 5

Inhibition of methylation blocked proliferation effect of Ad-Hiwi to Caco-2 or HT-29 cells. (a)–(d): DNA methylation levels in CACO-2 cells ((a), (b)) or HT-29 cells ((c), (d)) infected with 1 MOI Ad-RFP ((a), (c)) or with 1 MOI Ad-Hiwi ((b), (d)) after the treatment with 0, 0.05, 0.2, 0.8, or 2 μM DAC for 24 hours, respectively. (e) and (f): 2 μM DAC inhibited the Ad-Hiwi-promoted proliferation of Caco-2 or HT-29 cells by CCK-8 assay. Statistical significance was shown as , , and ; ns, no significance.

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