Site-Specific Secretome Map Evidences VSMC-Related Markers of Coronary Atherosclerosis Grade and Extent in the Hypercholesterolemic Swine

Figure 5

Representative photomicrographs of immunostained consecutive cross sections of RCA in a typical ATH case of HF group (immunopositive cells are dark brown). Immunostained sections demonstrate tissue codistribution (top row) and cell colocalization (middle and bottom rows) of anti-CHI3L1 with anti-αSM-actin and with anti-S100A4 antibodies in a fibrolipid plaque. Enlargements of plaque regions (black and red insets) are displayed in middle and bottom row, respectively. Plaque areas labelled by anti-αSM-actin, anti-S100A4, and anti-CHI3L1 antibodies are indicated (top panels, black arrows; bar = 200 μm) and colocalization of all three antibodies in the same cell type evidenced (bottom panels, black arrows; bar = 10 μm). CHI3L1 immunopositive cells resulted also in αSM-actin or S100A4 positive or both (middle panels, red arrows; bar = 20 μm).