Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer

Table 1

Methylation-specific primers of SNCA and FBN1.

Primer set	Forward primer	Reverse primer	Amp size (bp)	Annealing temperature (°C)
SNCA-M	CGGGTTGTAGCGTAGATTTC	CGTCGAATAACCACTCCC	125	53
SNCA-U	GTGTGGGTTGTAGTGTAGATTTT	TCATCAAATAACCACTCCCAA	129	53
FBN1-M	GTATTTTTTTCGCGAGAAATC	AATCGTAACCGCTACAACC	164	48
FBN1-U	AAAGTATTTTTTTTGTGAGAAATT	CCCAATCATAACCACTACAACC	170	48