Upregulated Expression of SOX4 Is Associated with Tumor Growth and Metastasis in Nasopharyngeal Carcinoma

Figure 5

Interference of SOX4 expression inhibited the migration of CNE2 cells. (a) CNE2 cells transfected with SOX4-siRNA or control siRNA were used for the wound-healing assay. Migration of cells to the wound was visualized at 0 and 48 h with a microscope (×200 magnification). (b) The histogram showed the relative wound width. The data are mean ± SEM (). (c) 1 × 10⁵ serum-free CNE2 cells which were transfected with SOX4-siRNA or control siRNA. After 16 h, representative images of the migrated cells were taken at the same magnification. (d) Absolute number of cells migrated through the transwell membrane. The member was counted in 10 fields under ×20 objective lens. (e) Western blot was used to investigate the changes of EMT markers in CNE2 cells transfected with SOX4-siRNA. (f) The bar demonstrated the expression ratio of the target protein to β-actin by densitometry. The data shown were representative of at least three independent experiments. Data were analyzed by Student’s -test. .

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