The Influence of Vitamin D Receptor Genetic Variants on Bone Mineral Density and Osteoporosis in Chinese Postmenopausal Women

Table 1

The PCR amplification and CRS-PCR analysis for investing VDR genetic variants.

SNPs	Primer sequences	PCR amplification fragment size (bp)	Annealing temperature (°C)	Region	Restriction enzyme	Genotype (bp)
p.Gly14Ala	5′-CACTTCCCTGCCTGACCCTA-3′; 5′-GTGAAAAATGCAAGGGCTCC-3′	213	59.8	Exon3	MaeI	GG: 195, 18; GC: 213, 195, 18; CC: 213
p.His305Gln	5′-ATTGTCTCTCACAGCCGGTCA-3′; 5′-GGCCCTCAGCAGGTCTTTGT-3′	206	61.4	Exon9	MaeIII	CC: 189, 17; CG: 206, 189, 17; GG: 206

Note: VDR: vitamin D receptor; SNPs: single nucleotide polymorphisms; PCR: polymerase chain reaction; CRS-PCR: created restriction site-PCR. Underlined nucleotides mark nucleotide mismatches enabling the use of the selected restriction enzymes for discriminating sequence variations.