Identification of Endogenous Controls for Analyzing Serum Exosomal miRNA in Patients with Hepatitis B or Hepatocellular Carcinoma

Figure 4

The stability of the candidate genes and the optimal number of reference genes for transcript normalization in subset 1. (a) The stabilities of the reference genes as determined by geNorm. (b) After excluding U6, miR-22^*, and miR-16 due to , we analyzed the pairwise variations using geNorm. The combination of miR-221, miR-103, let-7a, miR-181c, miR-181a, miR-191, and miR-26a (RG-7) was recommended. (c) The expression stability values were evaluated using NormFinder. (d) In NormFinder, the gene expression Acc.S.D. was analyzed. The lowest Acc.S.D. value indicated that the optimal reference gene set included miR-221 and miR-103 (RG-2).

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