Identification of Endogenous Controls for Analyzing Serum Exosomal miRNA in Patients with Hepatitis B or Hepatocellular Carcinoma

Figure 7

Effects of different reference genes on the normalization of miR-21 expression. The expression of miR-21 was measured in serum exosomes from 18 CHB patients, 18 HCC patients, and 18 healthy subjects by RT-qPCR. The difference in miR-21 expression between groups was analyzed by -test. represents the probability distribution for two groups of log₂-converted data that did not obey normal distribution; a nonparametric hypothesis test was used to identify their distribution. Different levels of statistical significance are denoted with asterisks (*). “ns” represents the statistics results indicating that the two groups of log₂-converted data were not significantly different.