Hyperoxia-Induced Protein Alterations in Renal Rat Tissue: A Quantitative Proteomic Approach to Identify Hyperoxia-Induced Effects in Cellular Signaling Pathways

Figure 2

(a) to (c) Comparison of the samples using a 2SD model (SD, standard deviation). Spots upregulated in the first sample of the comparison are depicted in yellow and downregulated spots are depicted in blue. Unregulated spots are shown as grey circles. The threshold spot volume for a regulation (>2SD <−2SD) is shown as grey lines. The red curve represents the frequency distribution of the log volume ratios. The blue curve represents a normalized model frequency fitted to the spot ratios so that the modal peak is zero. (a) Hyperoxia versus control at day 0. Log volume ratio (-axis) ranges from −1.0 to +1.0; number of spots (-axis) ranges from 0 to 420. (b) Hyperoxia versus control at day 3. Log volume ratio (-axis) ranges from −1.2 to +1.0; number of spots (-axis) ranges from 0 to 420. (c) Hyperoxia versus control at day 7. Log volume ratio (-axis) ranges from −1.5 to +1.9; number of spots (-axis) ranges from 0 to 320.

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