Quantitative ELISA-Like Immunohistochemistry of Fibroblast Growth Factor 23 in Diagnosis of Tumor-Induced Osteomalacia and Clinical Characteristics of the Disease
Table 2
Laboratory findings of the included 14 TIO cases.
Patient
Serum phosphate (mmol/L, reference 0.89–1.60)
Serum ALP (U/L, reference 0–130)
Serum calcium (mmol/L, reference 2.25–2.75)
Serum intact PTH (pmol/L, reference 1.60–6.90)
Urine phosphate (mmol/24 h)
Urine calcium (mmol/L, reference 2.5–7.5)
TmP/GFR (mmol/L, reference 0.80 to 1.35)
FGF23 expression (absorbance at 450 nm)
FGF23 RT-PCR
Case
0.34 ± 0.03
687.4 ± 54.0
2.35 ± 0.02
25.07
13.2
2.52
0.29
2.19 ± 0.31
Positive
Case
0.53 ± 0.18
231.1 ± 26.9
2.17 ± 0.05
7.55
15.5
1.17
0.45
0.68 ± 0.08
Poor mRNA quality
Case
0.51
65.4 ± 1.3
2.18 ± 0.11
6.70
30.5
1.60
0.36
1.97 ± 0.13
Poor mRNA quality
Case
0.38 ± 0.06
199.7 ± 4.4
2.30 ± 0.13
4.32
8.5
1.36
0.31
0.76 ± 0.09
Positive
Case
0.67 ± 0.03
471.0 ± 19.5
2.32
4.39
27.3
1.19
0.57
0.58 ± 0.03
Poor mRNA quality
Case
0.60 ± 0.09
162.8 ± 34.0
2.28 ± 0.09
7.48
21.6
1.20
0.56
1.02 ± 0.11
Positive
Case
0.61 ± 0.08
374.9 ± 21.5
2.11 ± 0.06
3.62
5.4
0.60
0.58
1.34 ± 0.1
Positive
Case
0.59 ± 0.10
323.2 ± 50.0
2.31 ± 0.09
5.38
26.4
7.28
0.58
0.93 ± 0.13
Positive
Case
0.45 ± 0.03
351 ± 12.7
2.14 ± 0.08
7.10
35.0
1.17
0.31
0.68 ± 0.12
Positive
Case
0.52 ± 0.10
333.2 ± 35.2
2.26 ± 0.03
9.42
15.6
2.48
0.45
0.67 ± 0.09
Positive
Case
0.55 ± 0.19
179.7 ± 14.8
2.15 ± 0.13
4.41
19.1
1.85
0.47
0.55 ± 0.07
Poor mRNA quality
Case
0.51 ± 0.05
141.4 ± 25.5
2.30 ± 0.11
3.99
16.0
1.18
0.44
0.49 ± 0.09
Poor mRNA quality
Case
0.50
64.1
2.37
NA
13.4
NA
0.45
0.67 ± 0.07
Positive
Case
0.49 ± 0.11
131.9
2.20 ± 0.18
14.36
20.2
1.00
0.42
0.50 ± 0.04
Poor mRNA quality
ALP: alkaline phosphatase; PTH: parathyroid hormone; TmP/GFR: maximum tubular resorption of phosphorus factored for glomerular filtration rate; FGF23: Fibroblast Growth Factor 23. FGF23 was measured by the quantitative ELISA-like immunohistochemistry from formalin-fixed and paraffin-embedded tissues. FGF23 PT-PCR was measured using the formalin-fixed and paraffin-embedded tissues.
