CYC1 Predicts Poor Prognosis in Patients with Breast Cancer

Figure 2

Knockdown of CYC1 expression inhibits breast cancer cells migration and invasion. The wound healing assay as monitored by optical microscopy in MDA-MB-231 and lower CYC1 expression, due to CYC1 siRNA, was confirmed by western blot (a). Quantization of migration assay showed the result of scratch assay in MDA-MB-435S, and lower expression of CYC1 in MDA-MB-435S cell was confirmed after treatment with CYC1 siRNA. (b). Transwell chamber invasion assay (c–f). Microscopic photograph of cell invasion after treatment with CYC1 siRNA in MDA-MB-231 (c) and MDA-MB-435S (e). Bar chart showed a statistical analysis of invasion assay in MDA-MB-231 (d) and MDA-MB-435S (f). .

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