Detection of OSR2, VAV3, and PPFIA3 Methylation in the Serum of Patients with Gastric Cancer

Table 1

Methylation-specific primers of OSR2, PPFIA3, and VAV3.

Primer set	Forward primer	Reverse primer	Annealing; temperature (°C)	Amp; size (bp)
OSR2-M	CGTAGCGCGTGGGATTTTAC	CCAATACTAACAAACCGAAACG	57	100
OSR2-U	GGTTTAGGAGGATGAAGTGT	CACCCTATAACCACCTTTCCCACA	58	85
VAV3-M	GGTTTTTTTTTCGCGCGGGATC	ACGAAAAACGCGCGAAACTCG	57	139
VAV3-U	GGTTTTTTTTTTGTGTGGGATT	CACAAAAAACACACAAAACTCA	57	140
PPFIA3-M	GGTATGTGGTCGTTTGTC	CGAATTACTAATACCGATCTCG	57	98
PPFIA3-U	GGTATGTGGTTGTTTGTT	CAAATTACTAATACCAATCTCA	54	98