Overexpression of GRK3, Promoting Tumor Proliferation, Is Predictive of Poor Prognosis in Colon Cancer

Figure 1

Analysis of GRK3 expression in tissues and cell lines of colon cancer. (a) Real-time quantitative polymerase chain reaction analysis of GRK3 expression in human colon cancer tissues and adjacent normal mucosa. Each relative GRK3 mRNA level was normalized using β-actin expression. indicate statistical significance between two groups. (b) Real-time PCR (left) and Western blot analyses (right) were performed to investigate GRK3 expression in human colon cancer cell lines. Data are given as mean ± SD of three independent experiments. Statistical comparisons were made using two-tailed unpaired t-test. , GRK3 expression in different colon cancer cell lines versus NCM460 cells.

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