Role of MMP-1 (-519A/G, -1607 1G/2G), MMP-3 (Lys45Glu), MMP-7 (-181A/G), and MMP-12 (-82A/G) Variants and Plasma MMP Levels on Obesity-Related Phenotypes and Microvascular Reactivity in a Tunisian Population

Table 1

Details of PCR primer sequences and RFLP conditions.

Gene	Polymorphism	SNP	Primer sequence	PCR conditions	Restriction enzyme
MMP-1	-519A/G	rs1144393	F: 5′-CATGGTGCTATCGCAATAGGGT-3′; R: 5′-TGCTACAGGTTTCTCCACACAC-3′	Initial denaturation of 3 min at 94°C, followed by 35 cycles of 45 s at 94°C, 1 min at 60°C, 45 s at 72°C, and a final extension at 72°C for 10 min	KpnI
MMP-1	-1607 1G/2G	rs1799750	F: 5′-TCGTGAGAATGTCTTCCCATT-3′; R: 5′-TCTTGGATTGATTTGAGATAAG; TGAAATC-3′	Initial denaturation of 2 min at 95°C, followed by 35 cycles of 45 s at 95°C, 1 min at 56°C, 45 s at 72°C, and a final extension at 72°C for 10 min	XmnI
MMP-3	Lys45Glu (A/G)	rs679620	F: 5′-CAACACCATAGCAGTAGCAGC-3′; R: 5′-CAGCCTCTCCTTCATACAGCC-3′	Initial denaturation of 3 min at 95°C, followed by 35 cycles of 1 min at 95°C, 45 s at 60°C, 45 s at 72°C, and a final extension at 72°C for 10 min	TaqI
MMP-7	-181A/G	rs11568818	F: 5′-TGGTACCATAATGTCCTGAATG-3′; R: 5′-TCGTTATTGGCAGGAAGCACACAATGAATT-3′	Initial denaturation of 3 min at 94°C, followed by 35 cycles of 45 s at 94°C, 1 min at 56°C, 45 s at 72°C, and a final extension at 72°C for 10 min	EcoRI
MMP-12	-82A/G	rs2276109	F: 5′-GAGATAGTCAAGGGATGATATCAGC-3′; R: 5′-AAGAGCTCCAGAAGCAGTGG-3′	Initial denaturation of 3 min at 94°C, followed by 35 cycles of 45 s at 94°C, 1 min at 60°C, 45 s at 72°C, and a final extension at 72°C for 10 min	PvuII

F: forward; MMP: matrix metalloproteinase; PCR: polymerase chain reaction; R: reverse; RLFP: restriction fragment length polymorphism; SNP: single nucleotide polymorphism.