Hsa_circ_0008309 May Be a Potential Biomarker for Oral Squamous Cell Carcinoma

Figure 4

(a) RT-qPCR analysis illustrating the hsa_circ_0008309 expression levels following transfection in SCC15 and CAL27 cell lines. Hsa_circ_0008309 was significantly overexpressed compared with the negative control. A Student’s t-test (two-tailed) was performed. Data are shown as the mean ± standard deviation, . (b) Overexpression of hsa_circ_0008309 significantly downregulates the expression of miR-136-5P and miR-382-5P compared with control in SCC15 and CAL27 cell lines by RT-qPCR. One-way ANOVA with Tukey’s post-hoc test was performed. Data are shown as the mean ± standard deviation, , . (c) Western blot analysis shows the ATXN1 protein level in SCC15 and CAL27 cell lines following transfection with hsa_circ_0008309 expression plasmid, miR-136-5P inhibitor plus hsa_circ_0008309 expression plasmid, miR-382-5P inhibitor plus hsa_circ_0008309 expression plasmid and a mock vector. GAPDH was used as an internal control. RT-qPCR: reverse transcription-quantitative polymerase chain reaction; ATXN1: Ataxin 1; miR: microRNA.

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