Moderate Fluid Shear Stress Could Regulate the Cytoskeleton of Nucleus Pulposus and Surrounding Inflammatory Mediators by Activating the FAK-MEK5-ERK5-cFos-AP1 Signaling Pathway

Table 1

Primer sequences for PCR.

Primers	Forward	Reverse	Length
Collagen II	5-TGGACGATCAGGCGAAACC-3′	5-GCTGCGGATGCTCTCAATCT-3	244 bp
Aggrecan	5-ACTCTGGGTTTTCGTGACTCT-3	5-ACACTCAGCGAGTTGTCATGG-3	80 bp
Cytokeratin 8	5-CAGAAGTCCTACAAGGTGTCCA-3	5-CTCTGGTTGACCGTAACTGCG-3	194 bp
MAP-1	5-AACATGAGCGAGTTGGTCAAG-3	5-GCTCGTAGATGTCCGCGAT-3	127 bp
MAP-2	5-CTCAGCACCGCTAACAGAGG-3	5-CATTGGCGCTTCGGACAAG-3	95 bp
MAP-4	5-CCGGGCCAAAGTAGAGAAAAA-3	5-GACTGAATATGGCTGTAGCTCAC-3	162 bp
IL-1β	5-ATGATGGCTTATTACAGTGGCAA-3	5-GTCGGAGATTCGTAGCTGGA-3	132 bp
TNF-α	5-CCTCTCTCTAATCAGCCCTCTG-3	5-GAGGACCTGGGAGTAGATGAG-3	220 bp
IL6	5-ACTCACCTCTTCAGAACGAATTG-3	5-CCATCTTTGGAAGGTTCAGGTTG-3	149 bp
IL8	5-TTTTGCCAAGGAGTGCTAAAGA-3	5-AACCCTCTGCACCCAGTTTTC-3	194 bp
MIP-1	5-CTGTGCTGATCCCAGTGAATC-3	5-TCAGTTCAGTTCCAGGTCATACA-3	61 bp
MCP-1	5-CAGCCAGATGCAATCAATGCC-3	5-TGGAATCCTGAACCCACTTCT-3	190 bp
NOS1	5-TTCCCTCTCGCCAAAGAGTTT-3	5-AAGTGCTAGTGGTGTCGATCT-3	118 bp
GAPDH	5-GGAGCGAGATCCCTCCAAAAT-3	5-GGCTGTTGTCATACTTCTCATGG-3	197 bp