Promoter Hypomethylation Is Responsible for Upregulated Expression of HAI-1 in Hepatocellular Carcinoma

Figure 3

HAI-1 expression is upregulated after demethylation in SMMC7721 cell line. (a) Chromatin condensation assay: SMMC7721 HCC cell line was stained with Hoechst 33342 after 5-Aza-dC treatment (0.5 μM to 10 μM) for 72 h. Arrow: the representative images of stained nuclei. . (b) Percentage of condensed nuclei is represented graphically (, , ). . Protein (c) and mRNA (d) levels of HAI-1 expression in the SMMC7721 cell line were upregulated in a dose-dependent manner after 5-Aza-dC treatment for three days and one day, respectively. (; , .) (e) Alternation of HAI-1 mRNA expression level in L02, immortal normal liver cell line, and HepG2 and SMMC7721, two HCC cell lines, with 10 μM 5-Aza-dC for 24 h (). .

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