Plasma microRNA-320c as a Potential Biomarker for the Severity of Knee Osteoarthritis and Regulates cAMP Responsive Element Binding Protein 5 (CREB5) in Chondrocytes
Table 1
Primer sequences used for vector construction and real-time PCR.
Gene
Primer sequence
miR-320c
F: AAAAGCTGGGTTGAGAGGGT
R: ACCCTCTCAACCCAGCTTTT
CREB5
F: CCCTGCCCAACCCTACAATG
R: GGACCTTGCATCCCCATGAT
CREB5 3′-UTR Wt
F: AGTCCAACCCTTTGCCTGAAA
R: TGACACCACAGCACAAACTCA
CREB5 3′-UTR Mut
F: CTTGGGAAACGCTTTGGTGC
R: TAGCTGACTGTACCTGGTTTAACA
c-jun promoter
F: GCAGCGGAGCATTACCTCAT
R: TAGCCCATGATGTCACCCCA
c-fos promoter
F: GGGGACATGCGTCTTCGC
R: CAAGGTGCTCCAGAGTGTGC
GAPDH
F: GGAGCGAGATCCCTCCAAAAT
R: GGCTGTTGTCATACTTCTCATGG
U6
F: GCTTCGGCAGCACATATACTAAAAT
R: CGCTTCACGAATTTGCGTGTCAT
F: forward; R: reverse; CREB5: cAMP responsive element binding protein 5; UTR: untranslated region; Wt: wild-type; and Mut: mutant.