Differential Downregulation of E-Cadherin and Desmoglein by Epidermal Growth Factor
Figure 4
Different kinetics for disruption of desmosomes and adherens junctions by EGF. (a) SCC 12F cells were treated with EGF for the indicated times, fixed, and then probed with E-cadherin or desmoglein-2 antibodies. Note the relocalization of the desmosomal cadherin desmoglein-2 from the cell borders following treatment with EGF for 4–6 h (white arrows). This pattern differs from that observed for the adherens junctional cadherin, E-cadherin, where strong border staining is evident at 6 hours post EGF treatment (white arrowheads). (b) Cells were treated with EGF, fixed, and then stained with phalloidin to stain the actin cytoskeleton, or with a pan-cytokeratin antibody, to label keratin filaments. Disorganization of the keratin network is seen at 6 hours (white arrow), while the actin cytoskeleton remains intact at 8 hours posttreatment (white arrowheads).
