Research Article

Differential Downregulation of E-Cadherin and Desmoglein by Epidermal Growth Factor

Figure 9

Effect of MMP inhibition on membrane localization of E-cadherin or desmoglein-2 after EGF treatment. Cells were serum-starved for 24 hours then treated with 50 μM of the broad spectrum MMP inhibitor GM6001X for 30 minutes before addition of 20 nM EGF for the indicated times. Cells were then fixed and probed with either an E-cadherin antibody that recognizes the intracellular epitope or desmoglein-2 antibody. White arrows indicate the internalized desmoglein-2 even in the presence of inhibitor, while the white arrowheads indicate the retention of E-cadherin at the cell-cell borders.
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