Review Article

Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin

Figure 2

Melanocytes culture. (a) 760 nm excitation wavelength: melanocytes appear as dendritic cells, with a clearly distinguishable cellular body (dark nucleus and fluorescent cytoplasm) and peripheral ramifications. (b) 820 nm excitation wavelength: increasing the excitation wavelength, the cellular body and the dendrites disappear. We can only notice the strong autofluorescence due to melanin granules inside the dendrites.
810749.fig.002a
(a)
810749.fig.002b
(b)