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Evidence-Based Complementary and Alternative Medicine
Volume 1 (2004), Issue 3, Pages 315-319
Original Article

Ozone Therapy on Cerebral Blood Flow: A Preliminary Report

1Department of Radiation Oncology, Dr Negrín Hospital Las Palmas (Canary Islands), Spain
2Department of Research Unit, Dr Negrín Hospital Las Palmas (Canary Islands), Spain
3Department of Radiology, Dr Negrín Hospital Las Palmas (Canary Islands), Spain
4Department of Medical Physics, Dr Negrín Hospital Las Palmas (Canary Islands), Spain
5La Paterna Medical Center Las Palmas (Canary Islands), Spain
6Department of Chronic Pain Unit, Dr Negrín Hospital Las Palmas (Canary Islands), Spain
7Canary Islands Institute for Cancer Research (ICIC), Las Palmas (Canary Islands), Spain

Received 29 February 2004; Accepted 20 August 2004

Copyright © 2004 Bernardino Clavo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Ozone therapy is currently being used in the treatment of ischemic disorders, but the underlying mechanisms that result in successful treatment are not well known. This study assesses the effect of ozone therapy on the blood flow in the middle cerebral and common carotid arteries. Seven subjects were recruited for the therapy that was performed by transfusing ozone-enriched autologous blood on 3 alternate days over 1 week. Blood flow quantification in the common carotid artery (n = 14) was performed using color Doppler. Systolic and diastolic velocities in the middle cerebral artery (n = 14) were estimated using transcranial Doppler. Ultrasound assessments were conducted at the following three time points: 1) basal (before ozone therapy), 2) after session #3 and 3) 1 week after session #3. The common carotid blood flow had increased by 75% in relation to the baseline after session #3 (P < 0.001) and by 29% 1 week later (P = 0.039). In the middle cerebral artery, the systolic velocity had increased by 22% after session #3 (P = 0.001) and by 15% 1 week later (P = 0.035), whereas the diastolic velocity had increased by 33% after session #3 (P < 0.001) and by 18% 1 week later (P = 0.023). This preliminary Doppler study supports the clinical experience of achieving improvement by using ozone therapy in peripheral ischemic syndromes. Its potential use as a complementary treatment in cerebral low perfusion syndromes merits further clinical evaluation.