Ethanol Extract of the Flower Chrysanthemum morifolium Augments Pentobarbital-Induced Sleep Behaviors: Involvement of Cl− Channel Activation
Figure 2
Effects of EFC on chloride influx in primary cultured cerebellar granule cells. After the culture of cerebellar granule cells for 8 days, the cells were incubated with MQAE overnight, and then EFC (1–4 μg/mL) and pentobarbital (PENT, 10 μM) were added 5 min prior to measurement. PENT: pentobarbital. Each column represents the mean with S.E.M. The significance of the effects of the compounds was assessed using analysis of variance (ANOVA). *, ***, compared to control.