Ganoderma lucidum Protects Dopaminergic Neuron Degeneration through Inhibition of Microglial Activation
Figure 8
GL protects against LPS or CF induced overexpression of mRNA levels of TNF-α (a) and IL-1β (b) in a dose-dependent fashion. Cultures were treated with GL at indicated concentration 30 min prior to exposure with 0.25 g/ml LPS or 150 g/ml CF. Total RNA was extracted and then subjected to real-time PCR as described in Methods section. Data are expressed as percentage of the control group (LPS or CF only treated group, respectively) calculated from the average threshold cycle values and presented as the mean ± SD. Independent RNA preparations from different sets of cultures were prepared and determinations were performed in triplicate from the RNA samples of a set of experiment. * compared with LPS or CF only treated cultures, respectively.
