Research Article

Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage

Figure 3

Expressions of three germ layer markers in RG-treated, differentiated cells. After 20 days of treatment, expression of early ectoderm, mesoderm, and endoderm markers were evaluated. RG-treated differentiated cells showed more dominant expression of mesodermal marker, Brachyury and endodermal marker, HNF3 . (a) Morphological observation of differentiating cells in suspension and attached state. (b) Expressions of three germ layer markers in reattached, RG-treated cells. (c) Immunostaining results of ectoderm (Nestin), mesoderm (Brachyury) and endoderm (HNF3 ).
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