Research Article
Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage
Table 1
Primer sequences used for RT-PCR and qPCR.
| | Gene | Forward | Reverse | bp |
| | Pluripotency | | | |
| | GAPDH |
GGCGTTCTCTTTGGAAAGGTGTTC |
GTACTCAGCGGCCAGCATCG | | | Oct4 |
GAGAACAATGAGAACCTTCAGGA |
CTCGAACCACATCCTTCTCT | | | Sox2 |
GCGTACGCAAATTAAAGTCCAGA |
TGCTATTCTTCGGCCAGTTG | | | Nanog |
GCGTACGCAAATTAAAGTCCAGA |
TGCTATTCTTCGGCCAGTTG | |
| | Differentiation | | | |
| | Brachyury |
TAAGGTGGATCTTCAGGTAGC |
CATCTCATTGGTGAGCTCCCT | 252 | | Cardiac actin |
TCTATGAGGGCTACGCTTTG |
CCTGACTGGAAGGTTAGATGG | 630 | | HNF3 |
CTACGCCAACATGAACTCCA |
GAGGTCCATGATCCACTGGT | 199 | | Nestin |
CAGCTGGCGCACCTCAAGATG |
AGGGAAGTTGGGCTCAGGACTGG | 209 | | Nkx2.5 |
CTTCAAGCCAGAGGCCTACG |
CCGCCTCTGTCTTCTTCAGC | 233 | | MHC |
GTCATTGCTGAAACCGAGAATG |
GCAAAGTACTGGATGACACGCT | 413 |
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