Research Article
Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage
Table 1
Primer sequences used for RT-PCR and qPCR.
| Gene | Forward | Reverse | bp |
| Pluripotency | | | |
| GAPDH |
GGCGTTCTCTTTGGAAAGGTGTTC |
GTACTCAGCGGCCAGCATCG | | Oct4 |
GAGAACAATGAGAACCTTCAGGA |
CTCGAACCACATCCTTCTCT | | Sox2 |
GCGTACGCAAATTAAAGTCCAGA |
TGCTATTCTTCGGCCAGTTG | | Nanog |
GCGTACGCAAATTAAAGTCCAGA |
TGCTATTCTTCGGCCAGTTG | |
| Differentiation | | | |
| Brachyury |
TAAGGTGGATCTTCAGGTAGC |
CATCTCATTGGTGAGCTCCCT | 252 | Cardiac actin |
TCTATGAGGGCTACGCTTTG |
CCTGACTGGAAGGTTAGATGG | 630 | HNF3 |
CTACGCCAACATGAACTCCA |
GAGGTCCATGATCCACTGGT | 199 | Nestin |
CAGCTGGCGCACCTCAAGATG |
AGGGAAGTTGGGCTCAGGACTGG | 209 | Nkx2.5 |
CTTCAAGCCAGAGGCCTACG |
CCGCCTCTGTCTTCTTCAGC | 233 | MHC |
GTCATTGCTGAAACCGAGAATG |
GCAAAGTACTGGATGACACGCT | 413 |
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