Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi

Figure 5

Flow cytometry analysis of T. cruzi epimastigotes treated with Et-Bra for 24 h and incubated with Rh123 and PI or AO. (a–d) Rh123 fluorescence intensity in: (a) control, (b) 100 μg mL⁻¹, (c) 150 μg mL⁻¹, (d) 200 μg mL⁻¹ Et-Bra. Rh123^high parasites were delimited by a marker (M1) and the corresponding percent of the cells included. (e) Average fluorescence peak values for the AO fluorescence, from at least three independent assays, showing a dose-dependent decrease after treatment with Et-Bra. Asterisks indicate 𝑃<.05 when compared to untreated epimastigotes.

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