Purification and the Secondary Structure of Fucoidanase from Fusarium sp. LD8

Figure 1

Gel filtration column chromatography. Chromatography of fucoidanase obtained from the column of Sephadex G-100 (2.5 × 100 cm). After the column had been washed well with 0.1 mol·L⁻¹ citric acid-citric sodium buffer (pH 6.0), it was eluted with 2000 mL of the same buffer solution at a flow rate of 0.33 mL·min⁻¹ with 6.6 mL elute in each fraction.