After 96 h of in vitro treatment the TNF-α concentration has shown a significant increase. After in vivo treatment, it was decreased the number of monocyte/macrophages cells (CD11b+), dendritic cells (CD11c+), granulocytes (Ly6G+), B lymphocytes (CD45R+), T lymphocytes (CD3+) and specifically in group 2, erythrocytes (TER-119+). Cell niches and cells with ring-shaped nuclei were present in the first 48 h, and after this diminished.