Treatment with at Homeopathic Complex Medication Modulates Mononuclear Bone Marrow Cell Differentiation
Figure 3
Microphotography of mononuclear bone marrow cells. Animals were treated in vivo and bone marrow cells were collected. Mononuclear cells were separated, concentrated to 2.5 × 105 cells/mL, plated on 24-well culture plates with glass coverslips as for the adherent cell experiments, and maintained at 37°C under 95% air/5% CO2 for 24, 48 and 72 h. Cells were stained with Giemsa. Original magnification: 40×. In (c) and (d), we show ring cells and in (e) and (f), we emphasize niches found in groups 2 and 3 after 48 h, respectively. In (a) it is possible to compare lymphocytes (Ly) and activated macrophages (Am) in the positive control (group 4) and in (b) (group 1; the negative control), resident macrophages (Rm) and active macrophages (Am) are seen (Bar = 10 Gm).
