Ellagic Acid, the Active Compound of Phyllanthus urinaria, Exerts In Vivo Anti-Angiogenic Effect and Inhibits MMP-2 Activity
Figure 4
Ellagic Acid inhibited the in vivo angiogenesis and MMP-2 activity induced on the CAMs of chicken embryos. (a) Representative photos of CAMs at the end of experiment. (b) Angiogenic index was quantified by counting the number of blood vessel branch points on each CAM. (c) Decreased MMP-2 activity in Ellagic Acid treated chorioallantoic memebranes. The protein extract was prepared by homogenizing the CAMs, centrifuging and using the resulting supernatant for gelatin zymography analysis. Bar value is the mean ± SD of three independent experiments. Asterisk means statistic significance in comparison with the vehicle control.
