Ellagic Acid, the Active Compound of Phyllanthus urinaria, Exerts In Vivo Anti-Angiogenic Effect and Inhibits MMP-2 Activity
Figure 5
(a) Phyllanthus urinaria extract dose-dependently inhibited the MMP-2 activity in HUVECs. Conditioned media taken from cultures of HUVECs, with or without the treatment of P. urinaria extract for 24 h, were used for the gelatin zymography analysis. To study the effects of P. urinaria extract on the mRNA expression of MMP in HUVECs, cells were treated with (b) P. urinaria for 24 h and the mRNA expressions of MMP-2, MMP-14, TIMP-2 and GAPDH were determined by RT-PCR analysis. (c) Cells treated with P. urinaria for 24 h were processed for western blotting to analyze the protein levels of MMP-2 in the conditioned media and cytosol.
