The Aqueous Soluble Polyphenolic Fraction of Psidium guajava Leaves Exhibits Potent Anti-Angiogenesis and Anti-Migration Actions on DU145 Cells

Figure 5

Effect of PE on expression of MMP-2 and MMP-9 in DU145 cells. DU145 cells (1 × 10⁷ cells) were treated with PE at concentrations (from A to E) 0, 0.1, 0.25, 0.5 and 1.0 mg ml⁻¹ respective (A), and for TIMP2 assay: DU145 cells (1 × 10⁷ cells) were seeded onto a 10 cm dish in the absence or the presence of PE at concentrations of 0.25 and 1.0 mg ml⁻¹, respectively. All cultures were incubated at 37°C for 48 h. The gelatin zymography showing the clear zones against the background reveals a dose responsive expression of both the matrix metalloproteinases MMP-2 and MMP-9 (a). For TIMP-2, an increased activity to 1.85- and 1.99-fold was indicated in the presence of 0.25 and 1.0 mg ml⁻¹ of PE, respectively (b).

(a)

(b)